ScienticAir Microbial Monitoring & 
Diagnose System Design

This equipment is mainly composed of several parts: air collector, peristaltic pump, vacuum pump, liquid preservation, chip reaction area and camera. A vacuum pump draws air into the sampling bottle. The peristaltic pump controls the entry of various liquids into the sampling bottle and the microfluidic chip. The liquid storage area is stored separately, containing the sample solution, ethanol solution, water, reaction solution (kept at a low temperature in the 4 degree refrigeration module). A peristaltic pump pumps the sample fluid into a collection bottle, and collects an air sample. This then enters the chip for analysis. The LAMP reaction process is photographed and the results are recorded by an embedded camera.

Sample Collection

We distribute the product to areas specifically vulnerable to epidemic initiation, be that densely populated areas such as restaurants or public transport stations, or places with unique interaction for cross-species contamination, such as stables, farms and animal markets.

Microfluidic chip technology

Here, pathogen cells are embedded into a solution by air pressure and the nucleic acid sequence released. This is purified through washing with ethanol against a selectively permeable membrane. Within the microfluidic chip, the purified sequence binds to specific primer sequences. The LAMP (amplification and DNA replication) allows for the colour change that we will measure.

RGB Modelling

A deep learning model was used to differentiate the speed and intensity of the final colour change from the LAMP reaction. This used a branch of computer vision - instance segmentation - which converted the visual data to numerical RGB values for computation. These were then fed as input into a convolutional neural network architecture implemented with PyTorch. We achieved up to 85% accuracy.

Result Analysis

We use experimentally determined and artificially learnt thresholds as the cutoff point, after which a positive warning is produced. That is, the quicker and more intense the colour change, the higher the initial concentration of bacteria, and above the threshold, it becomes dangerous.

Buffer Design

Multiple storage trials of lamp buffer D-2 and D were conducted at 4 degrees Celsius for 17 days using different primers and varying BSA concentrations.  All solutions remained viable.  The lystate and amplification reaction solutions were mixed.  It was concluded that there was no significant difference between buffer 5 minute and 20 minute in high bacterial concentration. Further testing is required.  During testing, we observed that the C5 LAMP was more effective than the C8 LAMP in detecting A3, B8, C5.  Our gaol was to determine the effectiveness of custom made LAMP buffer D-2 and phosphate free LAMP buffer in this process.

Components best optimized size

EcoFab Health+ offers a portfolio of technologically-advanced, energy efficient air diagnose and air purifier. Transform infectious disease forecasting, advanced analytics, and communication by engaging public, academic, and private sector performers.  Microfluidic and LAMP analytic technology, RGB modeling to provide dynamic automatic air diagnose to prevent infectious diseases outbreak. 

Microfluidic chip technology

RGB Model Generate Testing Result

RGB modelling: A deep learning model was used to differentiate the speed and intensity of the final colour change from the LAMP reaction. This used a branch of computer vision - instance segmentation - which converted the visual data to numerical RGB values for computation. These were then fed as input into a convolutional neural network architecture implemented with PyTorch. We achieved up to 85% accuracy.

Result Analysis

ScienticAir Microbial
Monitoring &
Diagonise Model

EcoFab Health+ offers a portfolio of technologically-advanced, energy efficient air diagnose and air purifier. Transform infectious disease forecasting, advanced analytics, and communication by engaging public, academic, and private sector performers.  Microfluidic and LAMP analytic technology, RGB modeling to provide dynamic automatic air diagnose to prevent infectious diseases outbreak.